Human NEIL1 (His Tag) recombinant protein

Human NEIL1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.

Endonuclease 8-like 1

ENDONUCLEASE VIII; NEI1; DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE NEIL1; FPG1; NEI HOMOLOG 1; ENDONUCLEASE 8-LIKE 1; 2810450N13RIK; DNA GLYCOSYLASE/AP LYASE NEIL1; ENDONUCLEASE VIII-LIKE 1; HFPG1; NEH1; NEI-LIKE PROTEIN 1

Q96FI4

NEIL1

E. coli

A DNA sequence encoding the human NEIL1 (AAH10876.1) (Met 1-Ser 390) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.

Human

The recombinant human NEIL1 comprises 400 amino acids and migrates as an approximately 45 kDa band as predicted in SDS-PAGE under reducing conditions.

> 84 % as determined by SDS-PAGE