NEIL1 antibody

NEIL1 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

NEIL1 Rabbit Polyclonal antibody. Positive WB detected in A375 cells. Observed molecular weight by Western-blot: 44kd

ELISA, WB

human, mouse, rat; other species not tested.

NEIL1 antibody; hFPG1 antibody; Nei homolog 1 antibody; NEI1 antibody; FPG1 antibody

Rabbit IgG

This antibody was obtained by immunization of NEIL1 recombinant protein (Accession Number: XM_006720681). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906) Read more about this antibody on the blog: http://blog.ptglab.com/index.php/proteintechs-anti-neil3-antibody-used-investigate-mechanisms-of-dna-repair/?preview=true

• Ma H, Wang J, Abdel-Rahman SZ, Hazra TK, Boor PJ, Khan MF. Induction of NEIL1 and NEIL2 DNA glycosylases in aniline-induced splenic toxicity. Toxicology and applied pharmacology. 251(1):1-7. 2011.