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Emerin antibody

Cat.#: 110237

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Product Information

  • Product Name
    Emerin antibody
  • Documents
  • Description
    Emerin Rabbit Polyclonal antibody. Positive IF detected in Hela cells, A431 cells, MCF-7 cells. Positive FC detected in HEK-293T cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in HeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF7 cells. Observed molecular weight by Western-blot: 35kd
  • Tested applications
    ELISA, WB, IP, IF, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    EDMD antibody; EMD antibody; Emerin antibody; LEMD5 antibody; STA antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Emerin recombinant protein (Accession Number: NM_000117). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110237(EMD antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110237(EMD antibody) at dilution of 1:1000

    IP result of EMD antibody (Catalog No:110237 for IP and Detection) with mouse skeletal muscle tissue lysate.

    IP result of EMD antibody (Catalog No:110237 for IP and Detection) with mouse skeletal muscle tissue lysate.

    IF result of anti-EMD(Catalog No:110237) in Hela cell by Dr. Ralph Kehlenbach.

    IF result of anti-EMD(Catalog No:110237) in Hela cell by Dr. Ralph Kehlenbach.

    Immunofluorescent analysis of Hela cells, using EMD antibody Catalog No:110237 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using EMD antibody Catalog No:110237 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HEK-293T cells were stained with 0.2ug EMD antibody (Catalog No:110237, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HEK-293T cells were stained with 0.2ug EMD antibody (Catalog No:110237, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.
  • References
    • Lenain C, Gusyatiner O, Douma S, van den Broek B, Peeper DS. Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence. Carcinogenesis. 36(11):1263-74. 2015.
    • Fu Y, Lv P, Yan G. MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells. Scientific reports. 5:17186. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"