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Product Name
ELAVL3 Polyclonal Antibody
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Description
Polyclonal antibody to ELAVL3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ELAVL3 antibody; HUC antibody; HUCL antibody; PLE21 antibody; ELAV-like protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELAVL3 (NP_001411.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ELAVL3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ELAVL3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunohistochemistry - ELAVL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ELAVL3 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ELAVL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using ELAVL3 antibody at dilution of 1:200 (40x lens).
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Background
Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3 (By similarity). May be involved in neuronal differentiation and maintenance.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"