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EIF6 antibody

Cat.#: 110270

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Product Information

  • Product Name
    EIF6 antibody
  • Documents
  • Description
    EIF6 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive WB detected in COLO 320 cells, A375 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue. Observed molecular weight by Western-blot: 27-30 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    b(2)gcn antibody; B(2)GCN homolog antibody; CAB antibody; eIF 6 antibody; EIF3A antibody; EIF6 antibody; gcn antibody; integrin beta 4 binding protein antibody; ITGB4BP antibody; p27(BBP) antibody; p27BBP antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of EIF6 recombinant protein (Accession Number: NM_001267810). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:110270(EIF6 antibody) at dilution of 1:1000

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:110270(EIF6 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110270(EIF6 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110270(EIF6 antibody) at dilution of 1:50 (under 10x lens)

  • Background
    p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), It functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.
  • References
    • Chendrimada TP, Finn KJ, Ji X. MicroRNA silencing through RISC recruitment of eIF6. Nature. 447(7146):823-8. 2007.
    • Sezgin G, Henson AL, Nihrane A. Impaired growth, hematopoietic colony formation, and ribosome maturation in human cells depleted of Shwachman-Diamond syndrome protein SBDS. Pediatric blood & cancer. 60(2):281-6. 2013.
    • Henson AL, Moore JB, Alard P, Wattenberg MM, Liu JM, Ellis SR. Mitochondrial function is impaired in yeast and human cellular models of Shwachman Diamond syndrome. Biochemical and biophysical research communications. 437(1):29-34. 2013.
    • Shi M, Liu Y, Feng L. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins. Evidence-based complementary and alternative medicine : eCAM. 2015:278014. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"