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Product Name
EIF5A2 antibody
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Description
EIF5A2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in mouse brain tissue, A2780 cells, mouse testis tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 17kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eIF 5A 2 antibody; EIF 5A2 antibody; EIF5A2 antibody; eIF5AII antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF5A2 recombinant protein (Accession Number: NM_020390). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110268(EIF5A2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110268(EIF5A2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110268(EIF5A2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-EIF5A2 (IP:Catalog No:110268, 3ug; Detection:Catalog No:110268 1:500) with mouse brain tissue lysate 4000ug.
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Background
Eukaryotic initiation factor 5A (EIF5A) plays an essential role in the viability of eukaryotic cells. EIF5A is known to act as a translation initiation factor specific for a small number of mRNAs, a cellular target of HIV-1 REV protein, and an exportin-4-dependent nuclear export cargo. It is also involved in mRNA turnover and the establishment of actin polarity. [PMID:16157662]. EIF5A2, one isoform of EIF5A, has a key at the level of mRNA turnoverby acting downstream of decapping. It also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity [PMID:14622290].
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References
- Liu Y, Du F, Chen W, Yao M, Lv K, Fu P. EIF5A2 is a novel chemoresistance gene in breast cancer. Breast cancer (Tokyo, Japan). 22(6):602-7. 2015.
- Xu G, Yu H, Shi X. Cisplatin sensitivity is enhanced in non-small cell lung cancer cells by regulating epithelial-mesenchymal transition through inhibition of eukaryotic translation initiation factor 5A2. BMC pulmonary medicine. 14:174. 2014.
- Jin Y, Liu Z, Cao W. Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression. PloS one. 7(2):e30419. 2012.
- Xu GD, Shi XB, Sun LB. Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells. Journal of Zhejiang University. Science. B. 14(6):460-7. 2013.
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