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Product Name
EIF3H Polyclonal Antibody
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Description
Polyclonal antibody to EIF3H
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
EIF3H antibody; EIF3S3 antibody; eIF3-gamma antibody; eIF3-p40 antibody; eukaryotic translation initiation factor 3 subunit H antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200 -
Validations
Western blot - EIF3H Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF3H antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - EIF3H Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using EIF3H antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - EIF3H Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug EIF3H antibody . Western blot was performed from the immunoprecipitate using EIF3H antibody at a dilition of 1:1000.
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Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
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