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Product Name
EIF2AK3 Monoclonal Antibody
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Description
Monoclonal antibody to EIF2AK3
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
EIF2AK3 antibody; PEK antibody; PERK antibody; WRS antibody; eukaryotic translation initiation factor 2-alpha kinase 3 antibody
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Isotype
Mouse IgG
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Preparation
Antigen: Recombinant protein of human EIF2AK3
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃ (regular) or -20℃ (long term). Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - EIF2AK3 Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF2AK3 antibody .Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"