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Product Name
EIF1AX Polyclonal Antibody
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Description
Polyclonal antibody to EIF1AX
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
EIF1AX antibody; EIF1A antibody; EIF1AP1 antibody; EIF4C antibody; eIF-1A antibody; eIF-4C antibody; eukaryotic translation initiation factor 1A, X-linked antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - EIF1AX Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF1AX antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - EIF1AX Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using EIF1AX antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF1AX Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using EIF1AX antibody at dilution of 1:100 (40x lens).
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Background
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"