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Product Name
EIF1AX antibody
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Description
EIF1AX Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human gliomas tissue. Positive WB detected in HeLa cells, A375 cells. Observed molecular weight by Western-blot: 16 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eIF 1A antibody; eIF 1A X isoform antibody; eIF 4C antibody; EIF1A antibody; EIF1AP1 antibody; EIF1AX antibody; EIF4C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF1AX recombinant protein (Accession Number: NM_001412). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110174(EIF1AX antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110174(EIF1AX antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:110174(EIF1AX Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.
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