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Product Name
EGR1 antibody
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Description
EGR1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human ovary tumor tissue, human cervical cancer tissue, human placenta tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 62 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
AT225 antibody; early growth response 1 antibody; EGR 1 antibody; EGR1 antibody; G0S30 antibody; KROX 24 antibody; KROX24 antibody; NGFI A antibody; TIS8 antibody; Transcription factor ETR103 antibody; Transcription factor Zif268 antibody; ZIF 268 antibody; Zinc finger protein 225 antibody; Zinc finger protein Krox 24 antibody; ZNF225 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001964). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110220(EGR1 Antibody) at dilution of 1:300
Immunofluorescent analysis of HeLa cells using Catalog No:110220(EGR1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-EGR1 (IP:Catalog No:110220, 4ug; Detection:Catalog No:110220 1:500) with mouse brain tissue lysate 2640ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:110220(EGR1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:110220(EGR1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Early growth response 1 (EGR1) is a member of EGR family which are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression. The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF. Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (Egr-site), thereby activating transcription of target genes whose products are required for mitogenesis and differentiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"