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Product Name
EEF1G Polyclonal Antibody
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Description
Polyclonal antibody to EEF1G
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
EEF1G antibody; EF1G antibody; GIG35 antibody; elongation factor 1-gamma antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human EEF1G
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - EEF1G Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EEF1G antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - EEF1G Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using EEF1G antibody (40x lens).
Immunohistochemistry - EEF1G Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using EEF1G antibody (40x lens).
Immunohistochemistry - EEF1G Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using EEF1G antibody (40x lens).
Immunohistochemistry - EEF1G Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using EEF1G antibody (40x lens).
Immunohistochemistry - EEF1G Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using EEF1G antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EEF1G Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using EEF1G antibody at dilution of 1:100 (40x lens).
Immunofluorescence - EEF1G Polyclonal Antibody
Immunofluorescence analysis of A431 cells using EEF1G antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Probably plays a role in anchoring the complex to other cellular components.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"