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Product Name
EEF1B2 antibody
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Description
EEF1B2 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse kidney tissue, SKOV-3 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 34kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EEF1B antibody; EEF1B1 antibody; EEF1B2 antibody; EF 1 beta antibody; EF1B antibody; Elongation factor 1 beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EEF1B2 recombinant protein (Accession Number: NM_021121). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110312(EEF1B2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110312(EEF1B2 antibody) at dilution of 1:100 (under 25x lens)
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Background
In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against full length EEF1B2 of human origin.
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References
- Mazan-Mamczarz K, Lal A, Martindale JL, Kawai T, Gorospe M. Translational repression by RNA-binding protein TIAR. Molecular and cellular biology. 26(7):2716-27. 2006.
- Mazan-Mamczarz K, Kawai T, Martindale JL, Gorospe M. En masse analysis of nascent translation using microarrays. BioTechniques. 39(1):61-2, 64, 66-7. 2005.
- Pitteri SJ, JeBailey L, Faça VM. Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery. PloS one. 4(11):e7916. 2009.
- Fofana B, Yao XH, Rampitsch C, Cloutier S, Wilkins JA, Nyomba BL. Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver. Proteomics. 10(3):417-34. 2010.
- Terui Y, Sakamoto A, Yoshida T. Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA. Amino acids. 47(2):345-56. 2015.
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