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Product Name
EEF1A2 antibody
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Description
EEF1A2 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, human brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 50-53kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eEF1A 2 antibody; EEF1A2 antibody; EEF1AL antibody; EF 1 alpha 2 antibody; EF1A antibody; Elongation factor 1 alpha 2 antibody; FLJ41696 antibody; HS1 antibody; Statin S1 antibody; STN antibody; STNL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EEF1A2 recombinant protein (Accession Number: NM_001958). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
WB results of Catalog No:110311(anti-EEF1A2) in different cells.
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110311(EEF1A2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110311(EEF1A2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-EEF1A2 (IP:Catalog No:110311, 4ug; Detection:Catalog No:110311 1:1000) with mouse brain tissue lysate 4000ug.
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Background
EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.
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References
- Scaggiante B, Dapas B, Bonin S. Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression. British journal of cancer. 106(1):166-73. 2012.
- Vui-Kee K, Mohd Dali AZ, Mohamed Rose I, Ghazali R, Jamal R, Mokhtar NM. Molecular markers associated with nonepithelial ovarian cancer in formalin-fixed, paraffin-embedded specimens by genome wide expression profiling. The Kaohsiung journal of medical sciences. 28(5):243-50. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"