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Product Name
EDAR antibody
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Description
EDAR Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive FC detected in HepG2 cells. Positive WB detected in A549 cells. Positive IP detected in A549 cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Downless homolog antibody; Ectodermal dysplasia receptor antibody; ectodysplasin A receptor antibody; ED1R antibody; ED3 antibody; ED5 antibody; EDA A1 receptor antibody; EDA A1R antibody; EDA1R antibody; EDA3 antibody; EDAR antibody; FLJ94390 antibody; HRM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EDAR recombinant protein (Accession Number: NM_022336). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:110298(EDAR antibody) at dilution of 1:1200
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110298(EDAR antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110298(EDAR antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-EDAR (IP:Catalog No:110298, 4ug; Detection:Catalog No:110298 1:500) with A549 cells lysate 2800ug.
1X10^6 HepG2 cells were stained with 0.2ug EDAR antibody (Catalog No:110298, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.
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References
- Cui CY, Yin M, Sima J. Involvement of Wnt, Eda and Shh at defined stages of sweat gland development. Development (Cambridge, England). 141(19):3752-60. 2014.
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