Product Name

ECM1 antibody

Documents

Description

ECM1 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, HepG2 cells, L02 cells, MCF7 cells, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, rat pancreas tissue. Positive IHC detected in human thyroid cancer tissue, human breast cancer tissue, human lung cancer tissue, human pancreas cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 50-55 kDa

Tested applications

ELISA, WB, IHC, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ECM1 antibody; extracellular matrix protein 1 antibody; Secretory component p85 antibody

Immunogen

Human ECM1 Recombinant protein (GST tag)

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ECM1 recombinant protein (Accession Number: NM_004425). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:50-1:500

Validations

A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:110294(ECM1 antibody) at dilution of 1:1500

Immunohistochemical of paraffin-embedded human thyroid cancer using Catalog No:110294(ECM1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human thyroid cancer using Catalog No:110294(ECM1 antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:110294(ECM1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis.ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(PMID:21128013) It inhibits MMP9 proteolytic activity. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies(PMID:21497598).

References

Sercu S, Lambeir AM, Steenackers E. ECM1 interacts with fibulin-3 and the beta 3 chain of laminin 332 through its serum albumin subdomain-like 2 domain. Matrix biology : journal of the International Society for Matrix Biology. 28(3):160-9. 2009.
Boylan KL, Andersen JD, Anderson LB, Higgins L, Skubitz AP. Quantitative proteomic analysis by iTRAQ(R) for the identification of candidate biomarkers in ovarian cancer serum. Proteome science. 8:31. 2010.
Merregaert J, Van Langen J, Hansen U. Phospholipid scramblase 1 is secreted by a lipid raft-dependent pathway and interacts with the extracellular matrix protein 1 in the dermal epidermal junction zone of human skin. The Journal of biological chemistry. 285(48):37823-37. 2010.
Chen H, Jia WD, Li JS. Extracellular matrix protein 1, a novel prognostic factor, is associated with metastatic potential of hepatocellular carcinoma. Medical oncology (Northwood, London, England). 28 Suppl 1:S318-25. 2011.
Gambichler T, Stücker M, Kreuter A. Expression of extracellular matrix proteins in reticular variant of mid-dermal elastolysis. Journal of the European Academy of Dermatology and Venereology : JEADV. 24(12):1481-4. 2010.
López-Marure R, Contreras PG, Dillon JS. Effects of dehydroepiandrosterone on proliferation, migration, and death of breast cancer cells. European journal of pharmacology. 660(2-3):268-74. 2011.
Gambichler T, Skrygan M, Czempiel V. Differential expression of connective tissue growth factor and extracellular matrix proteins in lichen sclerosus. Journal of the European Academy of Dermatology and Venereology : JEADV. 26(2):207-12. 2012.

ECM1 antibody

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