-
Product Name
E2F6 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to E2F6
-
Tested applications
WB, IHC, IF, IP, ChIP
-
Species reactivity
Human, Mouse
-
Alternative names
E2F6 antibody; E2F-6 antibody; transcription factor E2F6 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:100
CHIP 1:20 - 1:50 -
Validations
Western blot - E2F6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using E2F6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - E2F6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using E2F6 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - E2F6 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using E2F6 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - E2F6 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 3ug E2F6 antibody . Western blot was performed from the immunoprecipitate using E2F6 antibody at a dilition of 1:500.
Chromatin Immunoprecipitation - E2F6 Polyclonal Antibody
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using E2F6 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
-
Background
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"