-
Product Name
E2F4 antibody
- Documents
-
Description
E2F4 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HL-60 cells, Jurkat cells, NIH/3T3 cells, Raji cells. Positive IP detected in mouse brain tissue. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 60-70 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
E2F 4 antibody; E2F4 antibody; Transcription factor E2F4 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of E2F4 recombinant protein (Accession Number: NM_001950). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:110155(E2F4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:110155(E2F4 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-E2F4 (IP:Catalog No:110155, 3ug; Detection:Catalog No:110155 1:1000) with mouse brain tissue lysate 3600ug.
-
Background
Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein..The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.
-
References
- Valle BL, D'Souza T, Becker KG. Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells. PloS one. 8(4):e61836. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"