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Product Name
DYNC1I1 Polyclonal Antibody
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Description
Polyclonal antibody to DYNC1I1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
DYNC1I1 antibody; DNCI1 antibody; DNCIC1 antibody; cytoplasmic dynein 1 intermediate chain 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DYNC1I1 (NP_001129028.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:20 - 1:200 -
Validations
Western blot - DYNC1I1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DYNC1I1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - DYNC1I1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using DYNC1I1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DYNC1I1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using DYNC1I1 antibody at dilution of 1:100 (40x lens).
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Background
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"