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Product Name
Dynamitin antibody
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Description
Dynamitin Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, MCF7 cells, mouse brain tissue, mouse lung tissue. Positive IHC detected in human gliomas tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DCTN 50 antibody; DCTN2 antibody; DCTN50 antibody; dynactin 2 antibody; dynactin 2 (p50) antibody; Dynactin subunit 2 antibody; DYNAMITIN antibody; p50 dynamitin antibody; RBP50 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Dynamitin recombinant protein (Accession Number: NM_001261413). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:110133(dynactin-2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110133(dynactin-2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110133(dynactin-2 antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 1ug dynactin-2 antibody (Catalog No:110133, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the full length p50 dynamitin.
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