-
Product Name
DVL2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to DVL2
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
DVL2 antibody; dishevelled segment polarity protein 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DVL2 (NP_004413.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - DVL2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DVL2 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - DVL2 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using DVL2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"