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Product Name
DUSP1 / MKP1 Polyclonal Antibody
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Description
Polyclonal antibody to DUSP1 / MKP1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DUSP1 antibody; CL100 antibody; HVH1 antibody; MKP-1 antibody; MKP1 antibody; PTPN10 antibody; dual specificity phosphatase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human DUSP1 / MKP1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - DUSP1 / MKP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DUSP1 / MKP1 Antibody at 1:300 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .
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Background
Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"