Cart 2

DPP4 antibody

Cat.#: 110069

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    DPP4 antibody
  • Documents
  • Description
    DPP4 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human prostate cancer tissue. Positive WB detected in PC-3 cells. Observed molecular weight by Western-blot: 55-60 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human, hamster; other species not tested.
  • Alternative names
    ADABP antibody; ADCP 2 antibody; ADCP2 antibody; CD26 antibody; dipeptidyl peptidase 4 antibody; Dipeptidyl peptidase IV antibody; DPP IV antibody; DPP4 antibody; DPPIV antibody; T cell activation antigen CD26 antibody; TP103 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DPP4 recombinant protein (Accession Number: NM_001935). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110069(DPP4 antibody) at dilution of 1:500

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110069(DPP4 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110069(DPP4 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110069(DPP4 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of PC-3 cells using Catalog No:110069(DPP4 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of PC-3 cells using Catalog No:110069(DPP4 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    DPP4 is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is an intrinsic membrane glycoprotein anchored into the cell membrane by its N-terminal end. High levels of the enzyme are found in the brush-border membranes of the kidney proximal tubule and of the small intestine, but several other tissues also express the enzyme. The enzyme is present in the fetal colon but disappears at birth. It is ectopically expressed in some human colon adenocarcinomas and human colon cancer cell lines(PMID:1977364).The dimeric 150- 220 kDa DPPIV has been reported to be active and accessible to DFP labeling, but the 110 kDa monomeric DPPIV is not(PMID:9065413). Sometimes traces of the 290 kDa active dimeric form of DPP IV as well as a 55-60 kDa protein appeared in the immunopurified DPP IV preparation. N-terminal amino acid sequence analysis revealed that the 55-60 kDa protein represents a fragment of DPP IV starting at amino acid 28.(PMID:9654125).
  • References
    • Aroor AR, Sowers JR, Bender SB. Dipeptidylpeptidase inhibition is associated with improvement in blood pressure and diastolic function in insulin-resistant male Zucker obese rats. Endocrinology. 154(7):2501-13. 2013.
    • Jackson EK, Kochanek SJ, Gillespie DG. Dipeptidyl peptidase IV regulates proliferation of preglomerular vascular smooth muscle and mesangial cells. Hypertension. 60(3):757-64. 2012.
    • Nistala R, Habibi J, Aroor A. DPP4 inhibition attenuates filtration barrier injury and oxidant stress in the zucker obese rat. Obesity (Silver Spring, Md.). 22(10):2172-9. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"