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Product Name
DPH2 antibody
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Description
DPH2 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in human brain tissue, PC-3 cells. Positive IP detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 52kd
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DPH2 antibody; DPH2 homolog antibody; DPH2 homolog (S. cerevisiae) antibody; DPH2 like 2 antibody; DPH2L2 antibody; HsDph2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DPH2 recombinant protein (Accession Number: NM_001384). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110065(DPH2 antibody) at dilution of 1:400
IP Result of anti-DPH2 (IP:Catalog No:110065, 3ug; Detection:Catalog No:110065 1:1000) with mouse skeletal muscle tissue lysate 6000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110065(DPH2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:110065(DPH2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.
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