-
Product Name
DOCK9 antibody
- Documents
-
Description
DOCK9 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive IF detected in HeLa cells. Positive IP detected in mouse brain tissue. Positive WB detected in A549 cells, HeLa cells, human brain tissue, human heart tissue, human placenta tissue, MCF7 cells, mouse brain tissue. Observed molecular weight by Western-blot: 200-236 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
dedicator of cytokinesis 9 antibody; DKFZp686C11110 antibody; DKFZp686D2047 antibody; DKFZp686N04132 antibody; DOCK9 antibody; FLJ11949 antibody; FLJ16744 antibody; FLJ44528 antibody; FLJ45282 antibody; FLJ45601 antibody; KIAA1058 antibody; KIAA1085 antibody; ZIZ1 antibody; ZIZIMIN1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_015296). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:110048(DocK9 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:110048(DocK9 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:110048(DocK9 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:110048(DocK9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-DocK9 (IP:Catalog No:110048, 4ug; Detection:Catalog No:110048 1:800) with mouse brain tissue lysate 4000ug.
-
Background
DocK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"