DNMT3A antibody

DNMT3A antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

DNMT3A Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HepG2 cells, Jurkat cells, MCF7 cells, mouse testis tissue, NIH/3T3 cells, PC-3 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 120-130kd

ELISA, WB, IP

Human,Mouse,Rat; other species not tested.

DNA methyltransferase HsaIIIA antibody; DNA MTase HsaIIIA antibody; DNMT3A antibody; DNMT3A2 antibody; M.HsaIIIA antibody

Rabbit IgG

This antibody was obtained by immunization of DNMT3A recombinant protein (Accession Number: XM_017003526). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.