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Product Name
DiMethyl-DNMT3A-K44 pAb
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Description
Polyclonal antibody to DiMethyl-DNMT3A-K44
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Tested applications
IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
Dnmt3a antibody; MmuIIIA antibody; DNMT3A2 antibody; M.HsaIIIA antibody; TBRS antibody; DNA methyltransferase 3A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
IF 1:50 - 1:200
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Validations
Immunofluorescence - DiMethyl-DNMT3A-K44 pAb
Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - DiMethyl-DNMT3A-K44 pAb
Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - DiMethyl-DNMT3A-K44 pAb
Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - DiMethyl-DNMT3A-K44 pAb
Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
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