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Product Name
DNMT1 antibody
- Documents
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Description
DNMT1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 180-200 kDa
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Tested applications
ELISA, IP, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
AIM antibody; CXXC9 antibody; DNA MTase HsaI antibody; DNMT antibody; DNMT1 antibody; MCMT antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNMT1 recombinant protein (Accession Number: NM_001130823). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
IP Result of anti-DNMT1 (IP:Catalog No:109966, 5ug; Detection:Catalog No:109966 1:500) with HEK-293 cells lysate 2500ug.
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109966(DNMT1 Antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells using Catalog No:109966(DNMT1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
DNMT1 represents DNA (cytosine-5-)-methyltransferase 1
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References
- Li X, Sun Q, Li X. Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. European journal of nutrition. 54(7):1201-10. 2015.
- Hu Y, Sun Q, Li X. In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks. PloS one. 10(4):e0122643. 2015.
- Jia L, Li J, He B. Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries. Scientific reports. 6:19436. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"