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Product Name
DLST Polyclonal Antibody
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Description
Polyclonal antibody to DLST
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DLST antibody; DLTS antibody; dihydrolipoamide S-succinyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human DLST (NP_001924.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - DLST Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DLST antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - DLST Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using DLST antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DLST Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using DLST antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DLST Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using DLST antibody at dilution of 1:100 (40x lens).
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Background
Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex (By similarity). The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) (By similarity). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"