-
Product Name
DLD antibody
- Documents
-
Description
DLD Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, HepG2 cells, human brain tissue, mouse heart tissue. Positive IP detected in mouse liver tissue. Positive IHC detected in human kidney tissue, human brain tissue, human heart tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 56 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
dihydrolipoamide dehydrogenase antibody; DLD antibody; DLDH antibody; GCSL antibody; LAD antibody; PHE3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of DLD recombinant protein (Accession Number: NM_000108). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109987(DLD antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109987(DLD antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109987(DLD antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-DLD (IP:Catalog No:109987, 4ug; Detection:Catalog No:109987 1:1000) with mouse liver tissue lysate 6000ug.
-
Background
DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules: non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
-
References
- Carrozzo R, Torraco A, Fiermonte G. Riboflavin responsive mitochondrial myopathy is a new phenotype of dihydrolipoamide dehydrogenase deficiency. The chaperon-like effect of vitamin B2. Mitochondrion. 18:49-57. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"