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Product Name
DIS3 antibody
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Description
DIS3 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human ovary tumor tissue. Positive WB detected in HeLa cells, human brain tissue, mouse ovary tissue, mouse testis tissue. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bA555G22.1 antibody; DIS3 antibody; dis3p antibody; DKFZp667L1817 antibody; EXOSC11 antibody; FLJ10484 antibody; KIAA1008 antibody; Protein DIS3 homolog antibody; RP11 342J4.3 antibody; RRP44 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DIS3 recombinant protein (Accession Number: NM_014953). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109946(DIS3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109946(DIS3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109946(DIS3 antibody) at dilution of 1:100 (under 40x lens)
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Background
DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.
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References
- Chang HM, Triboulet R, Thornton JE, Gregory RI. A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway. Nature. 497(7448):244-8. 2013.
- Shi ZZ, Zhang YM, Shang L. Genomic profiling of rectal adenoma and carcinoma by array-based comparative genomic hybridization. BMC medical genomics. 5:52. 2012.
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