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Product Name
DIDO1 antibody
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Description
DIDO1 Rabbit Polyclonal antibody. Positive WB detected in human placenta tissue, mouse thymus tissue. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 59-70kd
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Tested applications
ELISA, WB, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BYE1 antibody; C20orf158 antibody; DATF 1 antibody; DATF1 antibody; death inducer obliterator 1 antibody; DIDO1 antibody; DIDO2 antibody; DIDO3 antibody; DIO 1 antibody; DIO1 antibody; dJ885L7.8 antibody; DKFZp434P1115 antibody; FLJ11265 antibody; hDido1 antibody; KIAA0333 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DIDO1 recombinant protein (Accession Number: BC000770). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:109941(DIDO1 antibody) at dilution of 1:800
Immunofluorescent analysis of HepG2 cells, using DIDO1 antibody Catalog No:109941 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug DIDO1 antibody (Catalog No:109941, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis. The immunogen of this antibody is the C-terminal of isoform 2 of DATF1(DIDO1).
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