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Product Name
DICER1 Polyclonal Antibody
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Description
Polyclonal antibody to DICER1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DICER1 antibody; DCR1 antibody; Dicer antibody; Dicer1e antibody; HERNA antibody; K12H4.8-LIKE antibody; MNG1 antibody; RMSE2 antibody; endoribonuclease Dicer antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:100 - 1:200 -
Validations
Western blot - DICER1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DICER1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - DICER1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DICER1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using DICER1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DICER1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using DICER1 antibody at dilution of 1:100 (40x lens).
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Background
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"