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Product Name
DIABLO antibody
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Description
DIABLO Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse testis tissue, Sp2/0 cells. Positive IHC detected in human testis tissue, human kidney tissue. Observed molecular weight by Western-blot: 20 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DIABLO antibody; diablo homolog (Drosophila) antibody; Diablo homolog antibody; mitOChondrial antibody; DIABLO S antibody; FLJ10537 antibody; FLJ25049 antibody; SMAC antibody; SMAC3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DIABLO recombinant protein (Accession Number: NM_001371333). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109936(DIABLO antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:109936(DIABLO antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:109936(DIABLO antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-DIABLO (IP:Catalog No:109936, 4ug; Detection:Catalog No:109936 1:500) with HEK-293 cells lysate 2000ug.
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Background
DIABLO, also named as SMAC, promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. This antibody recognizes all the three isoforms (27 kDa and 22 kDa) of DIABLO. The highest expression was in the testis, adrenal gland, and ears.
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