-
Product Name
DHX9 antibody
- Documents
-
Description
DHX9 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, mouse spleen tissue, mouse testis tissue, rat spleen tissue, rat testis tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 130 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
ATP dependent RNA helicase A antibody; DDX9 antibody; DEAH box protein 9 antibody; DHX9 antibody; LKP antibody; NDH II antibody; NDH2 antibody; NDHII antibody; Nuclear DNA helicase II antibody; RHA antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of DHX9 recombinant protein (Accession Number: BC014246). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109935(DHX9 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:109935(DHX9 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109935(DHX9 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using DHX9 antibody Catalog No:109935 at 1:100 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].
-
References
- Nowak JS, Choudhury NR, de Lima Alves F, Rappsilber J, Michlewski G. Lin28a regulates neuronal differentiation and controls miR-9 production. Nature communications. 5:3687. 2014.
- Choudhury NR, Nowak JS, Zuo J, Rappsilber J, Spoel SH, Michlewski G. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation. Cell reports. 9(4):1265-72. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"