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Product Name
DHX57 antibody
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Description
DHX57 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue. Positive WB detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 150 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DDX57 antibody; DEAH box protein 57 antibody; DHX57 antibody; FLJ32861 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DHX57 recombinant protein (Accession Number: NM_198963). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109934(DHX57 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109934(DHX57 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109934(DHX57 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109934(DHX57 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 (DHX57), also named as DDX57, is a member of the DEAH subfamily within the Dead box protein family which is involved in modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. This antibody reacts with 150 kDa DHX57 protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"