-
Product Name
DHX38 antibody
- Documents
-
Description
DHX38 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 141 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DDX38 antibody; DEAH box protein 38 antibody; DHX38 antibody; KIAA0224 antibody; PRP16 antibody; PRPF16 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of DHX38 recombinant protein (Accession Number: XM_011523484). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109933(DHX38 antibody) at dilution of 1:1000
IP Result of anti-DHX38 (IP:Catalog No:109933, 4ug; Detection:Catalog No:109933 1:700) with HeLa cells lysate 1400ug.
-
Background
The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.
-
References
- Zhe N, Wang J, Chen S. Heme oxygenase-1 plays a crucial role in chemoresistance in acute myeloid leukemia. Hematology (Amsterdam, Netherlands). 20(7):384-91. 2015.
- Ancelin K, Lange UC, Hajkova P. Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. Nature cell biology. 8(6):623-30. 2006.
- Durcova-Hills G, Tang F, Doody G, Tooze R, Surani MA. Reprogramming primordial germ cells into pluripotent stem cells. PloS one. 3(10):e3531. 2008.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"