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Product Name
DHX32 antibody
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Description
DHX32 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human kidney tissue. Positive IF detected in HeLa cells. Positive WB detected in mouse kidney tissue, A431 cells, COLO 320 cells, mouse colon tissue. Observed molecular weight by Western-blot: 84kd,75kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DDX32 antibody; DEAD/H box 32 antibody; DEAD/H helicase like protein 1 antibody; DEAH box protein 32 antibody; DHLP1 antibody; DHX32 antibody; FLJ10694 antibody; FLJ10889 antibody; HuDDX32 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DHX32 recombinant protein (Accession Number: XM_017016405). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109930(DHX32 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human testis using Catalog No:109930(DHX32 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:109930(DHX32 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:109930(DHX32 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DHX32, a new member of the DEAH subfamily of DEAD box proteins, is a novel putative RNA helicases with an activation-dependent paten of expression, and characterized by a conserved helicase domain consisting of 7 to 8 conserved motifs. RNA helicases of the DEAD box family is are highly conserved enzymes which are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DHX32 may play an important role in the development of colorectal cancer . This is a rabbit polyclonal anti-DHX32 antibody raised against part of C-terminal chain of human DHX32.
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