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Product Name
DHODH antibody
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Description
DHODH Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in MCF-7 cells. Positive IP detected in mouse spleen tissue. Positive WB detected in MCF7 cells, mouse ovary tissue, mouse spleen tissue, rat ovary tissue, rat spleen tissue. Observed molecular weight by Western-blot: 43kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DHOdehase antibody; DHODH antibody; dihydroorotate dehydrogenase antibody; Dihydroorotate oxidase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DHODH recombinant protein (Accession Number: NM_001361). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109919(DHODH antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109919(DHODH antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109919(DHODH antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using DHODH antibody Catalog No:109919 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-DHODH (IP:Catalog No:109919, 4ug; Detection:Catalog No:109919 1:1000) with mouse spleen tissue lysate 4000ug.
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Background
DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).
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