DHODH antibody

Cat.#: 109919

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    DHODH antibody
  • Documents
  • Description
    DHODH Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in MCF-7 cells. Positive IP detected in mouse spleen tissue. Positive WB detected in MCF7 cells, mouse ovary tissue, mouse spleen tissue, rat ovary tissue, rat spleen tissue. Observed molecular weight by Western-blot: 43kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DHOdehase antibody; DHODH antibody; dihydroorotate dehydrogenase antibody; Dihydroorotate oxidase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DHODH recombinant protein (Accession Number: NM_001361). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109919(DHODH antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109919(DHODH antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109919(DHODH antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109919(DHODH antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109919(DHODH antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109919(DHODH antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of MCF-7 cells, using DHODH antibody Catalog No:109919 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of MCF-7 cells, using DHODH antibody Catalog No:109919 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-DHODH (IP:Catalog No:109919, 4ug; Detection:Catalog No:109919 1:1000) with mouse spleen tissue lysate 4000ug.

    IP Result of anti-DHODH (IP:Catalog No:109919, 4ug; Detection:Catalog No:109919 1:1000) with mouse spleen tissue lysate 4000ug.

  • Background
    DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"