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Product Name
Desmin antibody
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Description
Desmin Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue. Positive IF detected in C2C12 cell. Positive IHC detected in human heart tissue, human appendix tissue, human colon tissue, human hysteromyoma tissue, human placenta tissue, human skeletal muscle tissue, human testis tissue, rat heart tissue. Positive FC detected in C2C12 cell. Observed molecular weight by Western-blot: 52-53 kDa
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Tested applications
ELISA, WB, IHC, IP, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CMD1I antibody; CSM1 antibody; CSM2 antibody; DES antibody; desmin antibody; FLJ12025 antibody; FLJ39719 antibody; FLJ41013 antibody; FLJ41793 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Desmin recombinant protein (Accession Number: NM_001927). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109897(DES antibody) at dilution of 1:4000
Immunohistochemical of paraffin-embedded human heart using Catalog No:109897(DES antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:109897(DES antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-Desmin (IP:Catalog No:109897, 4ug; Detection:Catalog No:109897 1:3000) with mouse heart tissue lysate 8000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed C2C12 cell using Catalog No:109897(Desmin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 C2C12 cell were stained with 0.2ug Desmin antibody (Catalog No:109897, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
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References
- Wang Z, Liu J, Sun W. Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy. Life sciences. 93(8):352-8. 2013.
- Su H, Wan Q, Tian XJ. MAD2B contributes to podocyte injury of diabetic nephropathy via inducing cyclin B1 and Skp2 accumulation. American journal of physiology. Renal physiology. 308(7):F728-36. 2015.
- Chen CJ, Cheng FC, Su HL. Improved neurological outcome by intramuscular injection of human amniotic fluid derived stem cells in a muscle denervation model. PloS one. 10(5):e0124624. 2015.
- Du J, Niu X, Wang Y. MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a. Scientific reports. 5:16163. 2015.
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