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Product Name
DEPDC6 antibody
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Description
DEPDC6 Rabbit Polyclonal antibody. Positive IHC detected in human thyroid cancer tissue. Positive WB detected in HeLa cells, COLO 320 cells, HT-1080 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
DEP domain containing 6 antibody; DEP.6 antibody; DEPDC6 antibody; Deptor antibody; DKFZp564B1778 antibody; FLJ12341 antibody; FLJ12428 antibody; FLJ13854 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DEPDC6 recombinant protein (Accession Number: NM_022783). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109891(DEPDC6 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human thyroid cancer slide using Catalog No:109891(DEPDC6 Antibody) at dilution of 1:50
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Background
DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"