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DDX50 antibody

Cat.#: 109836

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Product Information

  • Product Name
    DDX50 antibody
  • Documents
  • Description
    DDX50 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 83 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DDX50 antibody; DEAD box protein 50 antibody; Gu beta antibody; GU2 antibody; GUB antibody; Nucleolar protein Gu2 antibody; RH II/GuB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DDX50 recombinant protein (Accession Number: XM_017016626). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109836(DDX50 antibody) at dilution of 1:800

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109836(DDX50 antibody) at dilution of 1:800

    Immunofluorescent analysis of HepG2 cells, using DDX50 antibody Catalog No:109836 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using DDX50 antibody Catalog No:109836 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-DDX50 (IP:Catalog No:109836, 4ug; Detection:Catalog No:109836 1:800) with HeLa cells lysate 1200ug.

    IP Result of anti-DDX50 (IP:Catalog No:109836, 4ug; Detection:Catalog No:109836 1:800) with HeLa cells lysate 1200ug.

  • Background
    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box polypeptide 50 (DDX50, synonyms: GU2, GUB, MGC3199, RH-II/GuB) enzyme may be involved in ribosomal RNA synthesis or processing.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"