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Product Name
DDX43 antibody
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Description
DDX43 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue. Positive WB detected in HeLa cells, A375 cells, HepG2 cells, PC-3 cells. Observed molecular weight by Western-blot: 73 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cancer/testis antigen 13 antibody; CT13 antibody; DDX43 antibody; DEAD box protein 43 antibody; DEAD box protein HAGE antibody; DKFZp434H2114 antibody; HAGE antibody; Helical antigen antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDX43 recombinant protein (Accession Number: NM_018665). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109831(DDX43 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109831(DDX43 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109831(DDX43 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109831(DDX43 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DDX43 represents DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
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References
- Akiyama Y, Komiyama M, Miyata H. Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients. Oncology reports. 31(4):1683-90. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"