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Product Name
DDX3Y antibody
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Description
DDX3Y Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue. Positive IF detected in Hela cells. Positive IHC detected in human testis tissue.
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Tested applications
ELISA, IF, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DBY antibody; DDX3Y antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDX3Y recombinant protein (Accession Number: XM_047442775). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human testis using Catalog No:109829(DDX3Y antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using DDX3Y antibody Catalog No:109829 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:109829(DDX3Y Antibody) at dilution of 1:300
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Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX3Y is one member of DEAD box RNA helicases, encode by the gene locate on the chromosome Y in the azoospermia factor A region. Polyclonal anti-DDX3Y antibodies (14041-1-AP) are produced by immunizing animals with C-terminus of DDX3Y and detect an 73-kDa band. As DDX3Y has a structural homolog with DDX3X, the antibody can recognize both.
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