-
Product Name
DDX21 antibody
- Documents
-
Description
DDX21 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, BxPC-3 cells, COLO 320 cells, HEK-293 cells, HeLa cells, human testis tissue, K-562 cells, mouse colon tissue, mouse liver tissue, mouse testis tissue, rat liver tissue. Positive IP detected in COLO 320 cells. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 87 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
DDX21 antibody; DEAD box protein 21 antibody; DKFZp686F21172 antibody; Gu alpha antibody; GUA antibody; GURDB antibody; Nucleolar RNA helicase 2 antibody; Nucleolar RNA helicase Gu antibody; Nucleolar RNA helicase II antibody; RH II/GU antibody; RH II/GuA antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of DDX21 recombinant protein (Accession Number: NM_004728). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109821(DDX21 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109821(DDX21 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using DDX21 antibody Catalog No:109821 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-DDX21 (IP:Catalog No:109821, 4ug; Detection:Catalog No:109821 1:1000) with COLO 320 cells lysate 2800ug.
-
Background
DX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. Catalog# 10528-1-AP is a rabbit polyclonal antibody raised against N-terminal of human DDX21.
-
References
- Bonzheim I, Irmler M, Klier-Richter M. Identification of C/EBPβ target genes in ALK+ anaplastic large cell lymphoma (ALCL) by gene expression profiling and chromatin immunoprecipitation. PloS one. 8(5):e64544. 2013.
- Hirai Y, Louvet E, Oda T. Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21. Genes to cells : devoted to molecular & cellular mechanisms. 18(9):780-97. 2013.
- Ruppen I, Grau L, Orenes-Piñero E. Differential protein expression profiling by iTRAQ-two-dimensional LC-MS/MS of human bladder cancer EJ138 cells transfected with the metastasis suppressor KiSS-1 gene. Molecular & cellular proteomics : MCP. 9(10):2276-91. 2010.
- Salhia B, Van Cott C, Tegeler T. Differential effects of AKT1(p.E17K) expression on human mammary luminal epithelial and myoepithelial cells. Human mutation. 33(8):1216-27. 2012.
- Hochstatter J, Hölzel M, Rohrmoser M. Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA. The Journal of biological chemistry. 287(29):24365-77. 2012.
- Grau L, Luque-Garcia JL, González-Peramato P. A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness. PloS one. 8(1):e53328. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"