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Product Name
DDX20 antibody
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Description
DDX20 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells, mouse testis tissue. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 100 kDaa; 112-115 kDa
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Component of gems 3 antibody; DDX20 antibody; DEAD box protein 20 antibody; DEAD box protein DP 103 antibody; DKFZp434H052 antibody; DP103 antibody; Gemin 3 antibody; GEMIN3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDX20 recombinant protein (Accession Number: NM_007204). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109820(DDX20 antibody) at dilution of 1:500
IP Result of anti-DDX20 (IP:Catalog No:109820, 4ug; Detection:Catalog No:109820 1:1000) with HeLa cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109820(DDX20 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using DDX20 antibody Catalog No:109820 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with .2ug DDX20 antibody (Catalog No:109820, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
DDX20 represents DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
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References
- Shin EM, Hay HS, Lee MH. DEAD-box helicase DP103 defines metastatic potential of human breast cancers. The Journal of clinical investigation. 124(9):3807-24. 2014.
- Li Q, Xie J, He L. FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis. Bioscience reports. 35(6):. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"