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DDAH2 antibody

Cat.#: 109769

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Product Information

  • Product Name
    DDAH2 antibody
  • Documents
  • Description
    DDAH2 Rabbit Polyclonal antibody. Positive IP detected in COLO 320 cells. Positive WB detected in human heart tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 30-40 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DDAH antibody; DDAH 2 antibody; DDAH2 antibody; DDAHII antibody; Dimethylargininase 2 antibody; G6a antibody; NG30 antibody; Protein G6a antibody; S phase protein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DDAH2 recombinant protein (Accession Number: NM_001303008). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109769(DDAH2 antibody) at dilution of 1:500

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109769(DDAH2 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109769(DDAH2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109769(DDAH2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109769(DDAH2 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109769(DDAH2 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-DDAH2 (IP:Catalog No:109769, 3ug; Detection:Catalog No:109769 1:500) with COLO 320 cells lysate 2000ug.

    IP Result of anti-DDAH2 (IP:Catalog No:109769, 3ug; Detection:Catalog No:109769 1:500) with COLO 320 cells lysate 2000ug.

  • Background
    The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"