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Product Name
DCD Polyclonal Antibody
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Description
Polyclonal antibody to DCD
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
DCD antibody; AIDD antibody; DCD-1 antibody; DSEP antibody; HCAP antibody; PIF antibody; dermcidin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human DCD (NP_444513.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - DCD Polyclonal Antibody
Western blot analysis of extracts of mouse skin, using DCD antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 5s.
Immunofluorescence - DCD Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using DCD antibody . Blue: DAPI for nuclear staining.
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Background
DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues.; Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"