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Product Name
D2HGDH antibody
- Documents
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Description
D2HGDH Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive WB detected in human liver tissue, human heart tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
D2HGD antibody; D2HGDH antibody; FLJ42195 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of D2HGDH recombinant protein (Accession Number: NM_152783). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109817(D2HGDH antibody) at dilution of 1:500
IP result of anti-D2HGDH (Catalog No:109817 for IP and Detection) with mouse liver tissue lysate.
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109817(D2HGDH antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109817(D2HGDH antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-D2HGDH (IP:Catalog No:109817, 3ug; Detection:Catalog No:109817 1:500) with mouse liver tissue lysate 5000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:109817(D2HGDH Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.
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References
- Intlekofer AM, Dematteo RG, Venneti S. Hypoxia Induces Production of L-2-Hydroxyglutarate. Cell metabolism. 22(2):304-11. 2015.
- Krell D, Assoku M, Galloway M, Mulholland P, Tomlinson I, Bardella C. Screen for IDH1, IDH2, IDH3, D2HGDH and L2HGDH mutations in glioblastoma. PloS one. 6(5):e19868. 2011.
- Ma S, Jiang B, Deng W. D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget. 6(11):8606-20. 2015.
- Lin AP, Abbas S, Kim SW. D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2. Nature communications. 6:7768. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"