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Product Name
Cytokeratin 8 antibody
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Description
Cytokeratin 8 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, A431 cells, A549 cells, COLO 320 cells, HeLa cells, rat skin tissue. Positive FC detected in HepG2 cells, MCF-7 cells. Positive IF detected in MCF-7 cells, A431 cells, HepG2 cells. Positive IHC detected in human colon tissue, human breast cancer tissue, human placenta tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, FC, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CARD2 antibody; CK 8 antibody; CK8 antibody; CYK8 antibody; Cytokeratin 8 antibody; K2C8 antibody; keratin 8 antibody; KRT8 antibody; Type II keratin Kb8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cytokeratin 8 recombinant protein (Accession Number: NM_001256293). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109813(KRT8 antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109813(KRT8 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109813(KRT8 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of MCF-7 cells using Catalog No:109813(KRT8 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of MCF-7 cells using Catalog No:109813(KRT8 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug KRT8 antibody (Catalog No:109813, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT8 is often paired with keratin 18 in vivo.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"